Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
The samples collected from the players are sent to WADA’s laboratory in Montreal, Canada. The laboratory then performs two urine tests to detect the use of steroids. The human body produces testosterone and epitestosterone (which is another naturally occurring hormone), in nearly the same amounts, so their ratio is 1:1. The lab measures the quantities of testosterone and epitestosterone to make sure their ratio is nearly the same. In case, the ratio of testosterone is higher, a second urine test is conducted to confirm the presence of anabolic steroids.
In the end when we examine the various steroid drugs there is an important note we must discuss and to leave this out would be somewhat irresponsible. Anabolic androgenic steroids, while often referred to as drugs should not be viewed in the same typical light. Anabolic androgenic steroids are hormones, they are derived and based on hormones your body already naturally produces placing them in a bit of a unique category as compared to many of the other items you might find at your local RX. Of course the specific definitions can be a bit fuzzy at times, especially when we look at the manner in-which drugs are defined in the . Under . law drugs are defined as any substance that alters the body other than food from its normal function but guess what, alcohol is excluded from this rule and while anabolic androgenic steroids are a part of the normal human bodily function they are by no means excluded. Its a bit of a paradox as even the simplest minded can see.